新发表在美国的试验模型研究-脑外科专家顾建文教授-搜狐博客

我们有关大鼠颅脑创伤的新型模型发表在美国神经学杂志上，该工作经过团队近5年的大量工作积累而成。

Article title: Development of a rat model for studying blast-induced traumatic brain injury

Journal of the Neurological Sciences神经学杂志

Corresponding author（通讯作者）: Dr Jian-Wen Gu 顾建文

First author（第一作者）: Dr. Jing-Min Cheng 程敬民

Online publication complete: 16-MAY-2010
DOI information: 10.1016/j.jns.2010.04.010

SCI影响因子2.4

Development of a rat model for studying blast-induced traumatic brain injury

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Jingmin Cheng^a^,¹^,, JianwenGu^a^,^,, Yuan Ma^a^,²^,, Tao Yang^a^,²^,, Yongqin Kuang^a^,³^,, Bingcang Li^b^,⁴^,and Jianyi Kang^b^,⁵^,

^a Department of Neurosurgery, General Hospital of People's Liberation Army Chengdu Military Region, Chengdu, Sichuan 610083, China

^b State Key Laboratory of Trauma, Research Institute of Surgery, Research Institute for Traffic Medicine of People's Liberation Army, Daping Hospital, Third Military Medical University, Chongqing 400038, China

Received 31 August 2009;

revised 22 March 2010;

accepted 20 April 2010.

Available online 16 May 2010.

Abstract

Blast-induced traumatic brain injury (TBI) has been the predominant cause of neurotrauma in current military conflicts, and it is also emerging as a potential threat in civilian terrorism. The etiology of TBI, however, is poorly understood. Further study on the mechanisms and treatment of blast injury is urgently needed. We developed a unique rat model to simulate blast effects that commonly occur on the battlefield. An electric detonator with the equivalent of 400 mg TNT was developed as the explosive source. The detonator's peak overpressure and impulse of explosion shock determined the explosion intensity in a distance-dependent manner. Ninety-six male adult Sprague–Dawley rats were randomly divided into four groups: 5-cm, 7.5-cm, 10-cm, and control groups. The rat was fixed in a specially designed cabin with an adjustable aperture showing the frontal, parietal, and occipital parts of the head exposed to explosion; the eyes, ears, mouth, and nose were protected by the cabin. After each explosion, we assessed the physiologic, neuropathologic, and neurobehavioral consequences of blast injury. Changes of brain tissue water content and neuron-specific enolase (NSE) expression were detected. The results in the 7.5-cm group show that 87% rats developed apnea, limb seizure, poor appetite, and limpness. Diffuse subarachnoid hemorrhage and edema could be seen within the brain parenchyma, which showed a loss of integrity. Capillary damage and enlarged intercellular and vascular space in the cortex, along with a tattered nerve fiber were observed. These findings demonstrate that we have provided a reliable and reproducible blast-induced TBI model in rats.

Keywords: Blast; Traumatic brain injury; Animal model

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